The Presence of Ribulose 1,5-Diphosphate Carboxylase in the Nonphotosynthetic Endosperm of Germinating Castor Beans
نویسندگان
چکیده
منابع مشابه
The presence of ribulose 1,5-diphosphate carboxylase in the nonphotosynthetic endosperm of germinating castor beans.
Ribulose 1,5-diphosphate carboxylase was detected in extracts of germinating castor bean (Ricinus communis var. Hale) endosperms. This is the first report of this enzyme in a nonphotosynthetic (no chlorophyll) plant tissue. Radioactive 3-phosphoglyceric acid has been identified as the principle product resulting from the enzymatic condensation of (14)C-bicarbonate and ribulose-1,5-diP in endosp...
متن کاملDevelopment of ribulose 1,5-diphosphate carboxylase in nonphotosynthetic endosperms of germinating castor beans.
Ribulose 1,5-diphosphate (RuDP) carboxylase has been partially purified from dark-grown nonphotosynthetic endosperms of germinating castor beans (Ricinus communis var. Hale). The Km values for RuDP, HCO(3) (-), and Mg(2+) are 0.51, 33, and 1.78 mm, respectively. The pH optimum for the carboxylation reaction is pH 7.5. Germination is required for the development of the carboxylase in the endospe...
متن کاملLocalization and properties of ribulose diphosphate carboxylase from castor bean endosperm.
A substantial portion of the ribulose 1,5-diphosphate carboxylase activity in the endosperm of germinating castor beans (Ricinus communis var. Hale) is recovered in the proplastid fraction. The partially purified enzyme shows homology with the enzyme from spinach (Spinacia oleracea) leaves, as evidenced by its reaction against antibodies to the native spinach enzyme and to its catalytic subunit...
متن کاملDevelopment of ribulose-1,5-diphosphate carboxylase in castor bean cotyledons.
Light was not essential for the development of ribulose-1,5-diphosphate carboxylase protein or catalytic activity in the photosynthetic cotyledons of germinating castor beans (Ricinus communis). Cotyledons developing in the dark showed higher activity than those in the light. Returning cotyledons developing in the light to darkness resulted in a significant increase in ribulose-1,5-diphosphate ...
متن کاملThe breakdown of lipid reserves in the endosperm of germinating castor beans.
1. Lipid extracts were obtained from castor-bean endosperm tissue at various times during germination and, after purification, the total lipid content was determined. Quantitative measurements of the triglyceride and phospholipid content together with the fatty acid composition were made. 2. The total lipid content of the endosperm rapidly decreased during germination; after 10 days less than 2...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Physiology
سال: 1973
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.51.4.755